46 Sulfonation-Based Decontamination Alters Biotransformation and Excretion of Deoxynivalenol in Nursery Pigs

Abstract Deoxynivalenol (DON) is a primary mycotoxin in cereal feed ingredients that negatively affects intake and immune function of pigs. Formation DON sulfonate (DONS) with sulfites has become an effective approach to mitigate the toxicities practice, but metabolic fate under sulfonation-based decontamination was not well defined. In this study, 48 nursery pigs (35 d age, 10.8 ± 1.3 kg) were stratified by weight sex grouped following 2 x2 factorial design on content corn-soybean meal diets (1.2 vs. 4.1 ppm) inclusion NoTox Ultimate D, sulfite-based mitigant (0 0.25%). Four experimental diets, including low-DON no treatment (LD-NT); sulfonation (LD-ST): high-DON (HD-NT); (HD-ST), fed for 21 urine collection day 0, 10, fecal 21. Urine samples analyzed both targeted metabolite analysis untargeted metabolomic modeling. itself absent all samples, indicating complete biotransformation. As expected, led formation DONS feces correlation decrease two glucuronides (HD-ST < HD-NT). Interestingly, did concentrations deepoxy-deoxynivalenol (DOM) urine. Instead, it significantly increased DOM > The results indicate sulfite alters biotransformation excretion decreasing availability free absorption simultaneously increasing its microbial metabolism form DOM, inactivated metabolite, excretion.